Fluorescence microscopy--avoiding the pitfalls.

There was an error published in J. Cell Sci. 120, 1703-1705. On p. 1704, in the section ‘Image acquisition’, line 6 should read ‘all four pixels in a 2 2 square are summed into a single pixel’. For clarification, 2 2 binned pixels are twice the height and twice the width of the original pixel, but the pixel area is four times larger; similarly, for a 4 4 binned image the pixel height and width are four times larger, resulting in a pixel that has 16 times the area of the original pixels.